Crystallization of Polo-like kinase-1 via DARPin co-crystallization and via the SER approach
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Biochemistry Seminar
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last modified
Oct 23, 2009 03:51 PM
Roman C. Hillig Bayer Schering Pharma
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Nov 06, 2009 from 03:30 PM to 04:30 PM |
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Despite a multi length variant approach and an intensive screening campaign, the kinase domain of Polo-like kinase-1 initially resisted all crystallization efforts. Crystals were finally obtained via co-crystallization with a Plk1-specific DARPin developed for this purpose, and, in an alternative approach, via surface entropy reduction mutations. The resulting structures show that both approaches probably succeeded by modifying the same surface patch.